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Question
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
Options
Charge only
Size only
Charge to size ratio
All of the above
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Solution
In agarose gel electrophoresis, DNA molecules are separated on the basis of their size only.
Explanation:
The gel acts as a molecular sieve, where smaller DNA fragments migrate faster through the pores of the agarose matrix compared to larger ones. Although DNA molecules are negatively charged and move toward the positive electrode due to their charge, the separation principle hinges predominantly on size differences, not charge alone or charge-to-size ratio.
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