Advertisements
Advertisements
Question
Identify the activity of endonuclease and exonuclease in the given image.
Options
Endonuclease Exonuclease 

Endonuclease Exonuclease 

Endonuclease Exonuclease 
Endonuclease Exonuclease 

Advertisements
Solution
| Endonuclease | Exonuclease |
![]() |
![]() |
Explanation:
Endonucleases are a type of restriction enzyme that cuts the DNA at a specified location. It is widely used to clip the sequence in order to obtain DNA fragments with sticky ends. Sticky ends are single-stranded DNA segments that can establish hydrogen bonds with adjacent cut DNA segments. The enzyme ligase later joins these ends. Exonuclease is a restriction enzyme that removes nucleotides from the 5' and 3' ends of DNA strands.
APPEARS IN
RELATED QUESTIONS
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Distinguish between exonuclease and endonuclease.
How does restriction endonuclease function?
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
