Advertisements
Advertisements
Question
Identify the activity of endonuclease and exonuclease in the given image.
Options
Endonuclease Exonuclease 
Endonuclease Exonuclease 
Endonuclease Exonuclease 
Endonuclease Exonuclease 
Advertisements
Solution
| Endonuclease | Exonuclease |
|
|
Explanation:
Endonucleases are a type of restriction enzyme that cuts the DNA at a specified location. It is widely used to clip the sequence in order to obtain DNA fragments with sticky ends. Sticky ends are single-stranded DNA segments that can establish hydrogen bonds with adjacent cut DNA segments. The enzyme ligase later joins these ends. Exonuclease is a restriction enzyme that removes nucleotides from the 5' and 3' ends of DNA strands.
APPEARS IN
RELATED QUESTIONS
Explain with the help of a suitable example the naming of a restriction endonuclease.
How does restriction endonuclease function?
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
How are DNA fragments visualised once they are separated by gel electrophoresis?
