Advertisements
Advertisements
Question
Identify the activity of endonuclease and exonuclease in the given image.
Options
Endonuclease Exonuclease 

Endonuclease Exonuclease 

Endonuclease Exonuclease 
Endonuclease Exonuclease 

Advertisements
Solution
| Endonuclease | Exonuclease |
![]() |
![]() |
Explanation:
Endonucleases are a type of restriction enzyme that cuts the DNA at a specified location. It is widely used to clip the sequence in order to obtain DNA fragments with sticky ends. Sticky ends are single-stranded DNA segments that can establish hydrogen bonds with adjacent cut DNA segments. The enzyme ligase later joins these ends. Exonuclease is a restriction enzyme that removes nucleotides from the 5' and 3' ends of DNA strands.
APPEARS IN
RELATED QUESTIONS
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Molecular scissors, which cut DNA at specific site is ______.
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel

How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?
Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?
Why is it mandatory to cut both the vector DNA and the foreign source DNA with the exact same restriction enzyme when constructing recombinant DNA?
