Question

Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?

Options

• \[5'\text{—GAATTC—}3'\] and \[3'\text{—CTTAAG—}5'\]

• \[5'\text{—AAGCTT—}3'\] and \[3'\text{—TTCGAA—}5'\]

• \[5'\text{—GGATCC—}3'\] and \[3'\text{—CCTAGG—}5'\]

• \[5'\text{—GATATC—}3'\] and \[3'\text{—CTATAG—}5'\]

Answer 1

EcoRI specifically inspects and recognizes the palindromic sequence \[5'\text{—GAATTC—}3'\] / \[3'\text{—CTTAAG—}5'\], cleaving the sugar-phosphate backbones between the bases G and A on opposite strands.