Advertisements
Advertisements
Question
Why is it mandatory to cut both the vector DNA and the foreign source DNA with the exact same restriction enzyme when constructing recombinant DNA?
Options
To prevent ethidium bromide from staining non-recombinant plasmid molecules
To convert double-stranded vector DNA into linear single-stranded template DNA
To produce complementary sticky ends that can anneal and be joined end-to-end by DNA ligase
To eliminate the need for DNA ligase by facilitating spontaneous covalent bond formation
MCQ
Advertisements
Solution
Cutting both the foreign DNA and the vector DNA with the exact same restriction endonuclease ensures that both fragments have matching, complementary sticky ends that easily pair via hydrogen bonds and are covalently joined by DNA ligase.
shaalaa.com
Is there an error in this question or solution?
