Question

Why is it mandatory to cut both the vector DNA and the foreign source DNA with the exact same restriction enzyme when constructing recombinant DNA?

विकल्प

• To prevent ethidium bromide from staining non-recombinant plasmid molecules

• To convert double-stranded vector DNA into linear single-stranded template DNA

• To produce complementary sticky ends that can anneal and be joined end-to-end by DNA ligase

• To eliminate the need for DNA ligase by facilitating spontaneous covalent bond formation

Answer 1

Cutting both the foreign DNA and the vector DNA with the exact same restriction endonuclease ensures that both fragments have matching, complementary sticky ends that easily pair via hydrogen bonds and are covalently joined by DNA ligase.