Advertisements
Advertisements
प्रश्न
Identify the activity of endonuclease and exonuclease in the given image.
विकल्प
Endonuclease Exonuclease 
Endonuclease Exonuclease 
Endonuclease Exonuclease 
Endonuclease Exonuclease 
Advertisements
उत्तर
| Endonuclease | Exonuclease |
|
|
Explanation:
Endonucleases are a type of restriction enzyme that cuts the DNA at a specified location. It is widely used to clip the sequence in order to obtain DNA fragments with sticky ends. Sticky ends are single-stranded DNA segments that can establish hydrogen bonds with adjacent cut DNA segments. The enzyme ligase later joins these ends. Exonuclease is a restriction enzyme that removes nucleotides from the 5' and 3' ends of DNA strands.
APPEARS IN
संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Mention the difference in the mode of action of exonuclease and endonuclease.
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?
Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?
