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प्रश्न
How are DNA fragments visualised once they are separated by gel electrophoresis?
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उत्तर
The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation (you cannot see pure DNA fragments in the visible light and without staining). You can see bright orange coloured bands of DNA in a ethidium bromide stained gel exposed to UV light.
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संबंधित प्रश्न
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Do eukaryotic cells have restriction endonucleases? Justify your answer.
Explain briefly:
Restriction enzymes and DNA
Distinguish between exonuclease and endonuclease.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Restriction enzymes ______.
Molecular scissors, which cut DNA at specific site is ______.
Would you choose an exonuclease while producing a recombinant DNA molecule?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
