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प्रश्न
How are DNA fragments visualised once they are separated by gel electrophoresis?
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उत्तर
The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation (you cannot see pure DNA fragments in the visible light and without staining). You can see bright orange coloured bands of DNA in a ethidium bromide stained gel exposed to UV light.
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संबंधित प्रश्न
Explain briefly:
Restriction enzymes and DNA
How does restriction endonuclease function?
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
'Restriction' in restriction enzyme refers to
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
Which of the following statements does not hold true for restriction enzyme?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?
What is the origin of agarose, the matrix most commonly used in gel electrophoresis?
