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प्रश्न
State the principle involved in separation of DNA fragments using gel electrophoresis.
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उत्तर
As negatively charged molecules, DNA fragments can be forced to migrate in the direction of the anode through a medium or matrix in order to be separated. Through the sifting effect that the agarose gel provides, the DNA fragments separate (resolve) according to their size. Therefore, the size of the fragment determines how far it travels.
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
'Restriction' in Restriction enzyme refers to ______.
Would you choose an exonuclease while producing a recombinant DNA molecule?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of ______.
