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प्रश्न
State the principle involved in separation of DNA fragments using gel electrophoresis.
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उत्तर
As negatively charged molecules, DNA fragments can be forced to migrate in the direction of the anode through a medium or matrix in order to be separated. Through the sifting effect that the agarose gel provides, the DNA fragments separate (resolve) according to their size. Therefore, the size of the fragment determines how far it travels.
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
Which of the following radioisotope is not suitable for DNA labeling based studies?
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
Would you choose an exonuclease while producing a recombinant DNA molecule?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
