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प्रश्न
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
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उत्तर
If the restriction enzymes would cut DNA at random sites instead of at specific sites, then the DNA fragments obtained will not have ‘sticky ends’. In the absence of sticky ends, construction of recombinant DNA molecules would not be possible.
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संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Explain briefly:
Restriction enzymes and DNA
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Restriction enzymes ______.
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DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
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CTTAAG
GAATTC
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- What is their significance in biotechnology?
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Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?
How are separated DNA bands visualized following agarose gel electrophoresis?
Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?
