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प्रश्न
How does one visualise DNA on an agarose gel?
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उत्तर
A compound called Ethidium Bromide stains DNA, which on irradiating with Ultraviolet, fluoresce gives orange light. Hence, DNA fragments appear as orange band in the presence of Ethidium Bromide and UV.
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संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Mention the difference in the mode of action of exonuclease and endonuclease.
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Distinguish between exonuclease and endonuclease.
How does restriction endonuclease function?
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Give a reason why :
Single cloning site is preferred in a vector.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
Would you choose an exonuclease while producing a recombinant DNA molecule?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
Identify the activity of endonuclease and exonuclease in the given image.
How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?
According to the sieving effect of an agarose gel matrix, which DNA fragments move the farthest and fastest from the loading wells?
Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?
