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प्रश्न
How does one visualise DNA on an agarose gel?
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उत्तर
A compound called Ethidium Bromide stains DNA, which on irradiating with Ultraviolet, fluoresce gives orange light. Hence, DNA fragments appear as orange band in the presence of Ethidium Bromide and UV.
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संबंधित प्रश्न
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Do eukaryotic cells have restriction endonucleases? Justify your answer.
Explain briefly:
Restriction enzymes and DNA
Distinguish between exonuclease and endonuclease.
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
Give a reason why :
Single cloning site is preferred in a vector.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
Which of the following radioisotope is not suitable for DNA labeling based studies?
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
DNA fragments separate according to size through?
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
While isolating DNA from bacteria, which of the following enzymes is not required?
Which of the following bacteria is not a source of restriction endonuclease?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
State the importance of elution in this process.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
State the principle involved in separation of DNA fragments using gel electrophoresis.
