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प्रश्न
How does one visualise DNA on an agarose gel?
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उत्तर
A compound called Ethidium Bromide stains DNA, which on irradiating with Ultraviolet, fluoresce gives orange light. Hence, DNA fragments appear as orange band in the presence of Ethidium Bromide and UV.
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संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Suggest a technique to a researcher who needs to separate fragments of DNA.
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
State the importance of elution in this process.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of ______.
Which of the following was the very first restriction endonuclease to be isolated and characterized?
In the restriction endonuclease EcoRI, what do the letter 'R' and the Roman numeral 'I' represent?
Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?
Why is it mandatory to cut both the vector DNA and the foreign source DNA with the exact same restriction enzyme when constructing recombinant DNA?
