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प्रश्न
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
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उत्तर
The palindromic sequences i.e. the sequence of base pairs read the same on both the DNA strands when the orientation of reading is kept the same, eg :
5' —— GAATTC —— 3'
3' —— CTTAAG —— 5'
- Every endonuclease inspects the entire DNA sequence for the palindromic recognition sequence.
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
Which of the following statements does not hold true for restriction enzyme?
Would you choose an exonuclease while producing a recombinant DNA molecule?
How does one visualise DNA on an agarose gel?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
State the importance of elution in this process.
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
