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प्रश्न
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
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उत्तर
The palindromic sequences i.e. the sequence of base pairs read the same on both the DNA strands when the orientation of reading is kept the same, eg :
5' —— GAATTC —— 3'
3' —— CTTAAG —— 5'
- Every endonuclease inspects the entire DNA sequence for the palindromic recognition sequence.
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संबंधित प्रश्न
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
Which of the following radioisotope is not suitable for DNA labeling based studies?
DNA fragments separate according to size through?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
