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प्रश्न
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
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उत्तर
The palindromic sequences i.e. the sequence of base pairs read the same on both the DNA strands when the orientation of reading is kept the same, eg :
5' —— GAATTC —— 3'
3' —— CTTAAG —— 5'
- Every endonuclease inspects the entire DNA sequence for the palindromic recognition sequence.
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संबंधित प्रश्न
How does restriction endonuclease function?
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
Which of the following radioisotope is not suitable for DNA labeling based studies?
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
What is elution?
State the principle involved in separation of DNA fragments using gel electrophoresis.
