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प्रश्न
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.
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उत्तर
In DNA, a palindrome is a sequence of base pairs that reads the same on both strands when the reading orientation is constant.
| (i) | Eco RI: | 5' G A A T T C 3' |
| 3' C T T A A G 5' | ||
| (ii) | Hind III: | 5' A A G C T T 3' |
| 3' T T C G A A 5' | ||
| (iii) | Bam HI: | 5' G G A T C C 3' |
| 3' C CT A G G 5' | ||
| (iv) | Sal I: | 5' G T C G A C 3' |
| 3' C A G C T G 5' | ||
| (v) | Pst I: | 5' C T G C A G 3' |
| 3' G A C G T C 5' |
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संबंधित प्रश्न
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Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
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Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
DNA fragments separate according to size through?
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
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'Restriction' in Restriction enzyme refers to ______.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
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Would you choose an exonuclease while producing a recombinant DNA molecule?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
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Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
State the importance of elution in this process.
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
How are DNA fragments visualised once they are separated by gel electrophoresis?
Identify the activity of endonuclease and exonuclease in the given image.
