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प्रश्न
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.
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उत्तर
In DNA, a palindrome is a sequence of base pairs that reads the same on both strands when the reading orientation is constant.
| (i) | Eco RI: | 5' G A A T T C 3' |
| 3' C T T A A G 5' | ||
| (ii) | Hind III: | 5' A A G C T T 3' |
| 3' T T C G A A 5' | ||
| (iii) | Bam HI: | 5' G G A T C C 3' |
| 3' C CT A G G 5' | ||
| (iv) | Sal I: | 5' G T C G A C 3' |
| 3' C A G C T G 5' | ||
| (v) | Pst I: | 5' C T G C A G 3' |
| 3' G A C G T C 5' |
APPEARS IN
संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Mention the difference in the mode of action of exonuclease and endonuclease.
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Do eukaryotic cells have restriction endonucleases? Justify your answer.
Explain briefly:
Restriction enzymes and DNA
Distinguish between exonuclease and endonuclease.
How does restriction endonuclease function?
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Which of the following radioisotope is not suitable for DNA labeling based studies?
Restriction enzymes ______.
'Restriction' in restriction enzyme refers to
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
'Restriction' in Restriction enzyme refers to ______.
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
Which of the following statements does not hold true for restriction enzyme?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
How does one visualise DNA on an agarose gel?
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
