Advertisements
Advertisements
प्रश्न
How does restriction endonuclease function?
Advertisements
उत्तर
Each restriction endonuclease finds its specific palindromic nucleotide sequences in the DNA and cuts the DNA at these specific sites. It does so by binding to the DNA at these sites and cutting both the strands at specific points in their sugar-phosphate backbones.
APPEARS IN
संबंधित प्रश्न
Give a reason why :
Single cloning site is preferred in a vector.
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
'Restriction' in restriction enzyme refers to
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
'Restriction' in Restriction enzyme refers to ______.
While isolating DNA from bacteria, which of the following enzymes is not required?
Which of the following statements does not hold true for restriction enzyme?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
State the principle involved in separation of DNA fragments using gel electrophoresis.
