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प्रश्न
How does restriction endonuclease function?
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उत्तर
Each restriction endonuclease finds its specific palindromic nucleotide sequences in the DNA and cuts the DNA at these specific sites. It does so by binding to the DNA at these sites and cutting both the strands at specific points in their sugar-phosphate backbones.
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संबंधित प्रश्न
Suggest a technique to a researcher who needs to separate fragments of DNA.
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
How does one visualise DNA on an agarose gel?
