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प्रश्न
How does restriction endonuclease function?
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उत्तर
Each restriction endonuclease finds its specific palindromic nucleotide sequences in the DNA and cuts the DNA at these specific sites. It does so by binding to the DNA at these sites and cutting both the strands at specific points in their sugar-phosphate backbones.
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संबंधित प्रश्न
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Which of the following radioisotope is not suitable for DNA labeling based studies?
Restriction enzymes ______.
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
State the importance of elution in this process.
How are DNA fragments visualised once they are separated by gel electrophoresis?
Which of the following was the very first restriction endonuclease to be isolated and characterized?
Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?
