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प्रश्न
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
पर्याय
Endonuclease
Exonuclease
DNA ligase
Hind - II
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उत्तर
Exonuclease
Explanation:
The enzyme that catalyzes the removal of nucleotides from the ends of DNA is exonuclease. Exonuclease specifically removes nucleotides one by one from the ends of a DNA strand, whereas endonucleases cut DNA at internal sites. DNA ligase joins DNA fragments but does not remove nucleotides. Hind II is a type of restriction endonuclease that cuts DNA at specific internal sequences, not at the ends.
संबंधित प्रश्न
How does restriction endonuclease function?
Answer the following question.
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Give a reason why :
Single cloning site is preferred in a vector.
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There is a restriction endonudease called as EcoRI. What does co part in it stands for?
Molecular scissors, which cut DNA at specific site is ______.
DNA fragments separate according to size through?
'Restriction' in Restriction enzyme refers to ______.
While isolating DNA from bacteria, which of the following enzymes is not required?
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
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Which of the following statements does not hold true for restriction enzyme?
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A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
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\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
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- Explain the convention for naming EcoRI.
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State the principle involved in separation of DNA fragments using gel electrophoresis.
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