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प्रश्न
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
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उत्तर
- Anode = S
- Smallest/lightest DNA Strand = R
- Agarose gel = T
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संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
'Restriction' in Restriction enzyme refers to ______.
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
Identify the activity of endonuclease and exonuclease in the given image.
