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प्रश्न
What is elution?
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उत्तर
The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.
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संबंधित प्रश्न
Mention the difference in the mode of action of exonuclease and endonuclease.
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Which of the following radioisotope is not suitable for DNA labeling based studies?
Restriction enzymes ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
Which of the following bacteria is not a source of restriction endonuclease?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
State the principle involved in separation of DNA fragments using gel electrophoresis.
Identify the activity of endonuclease and exonuclease in the given image.
