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प्रश्न
What is elution?
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उत्तर
The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.
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संबंधित प्रश्न
Explain briefly:
Restriction enzymes and DNA
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
'Restriction' in Restriction enzyme refers to ______.
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
State the principle involved in separation of DNA fragments using gel electrophoresis.
Which of the following was the very first restriction endonuclease to be isolated and characterized?
How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?
How are separated DNA bands visualized following agarose gel electrophoresis?
