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प्रश्न
What is elution?
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उत्तर
The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.
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संबंधित प्रश्न
How does a restriction nuclease function? Explain
Explain briefly:
Restriction enzymes and DNA
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
'Restriction' in restriction enzyme refers to
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
While isolating DNA from bacteria, which of the following enzymes is not required?
Which of the following statements does not hold true for restriction enzyme?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
