Advertisements
Advertisements
Question
What is elution?
Advertisements
Solution
The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.
APPEARS IN
RELATED QUESTIONS
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Mention the difference in the mode of action of exonuclease and endonuclease.
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
Restriction enzymes ______.
Molecular scissors, which cut DNA at specific site is ______.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.

(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.

What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
