Advertisements
Advertisements
Question
What is elution?
Advertisements
Solution
The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.
APPEARS IN
RELATED QUESTIONS
Explain briefly:
Restriction enzymes and DNA
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
Restriction enzymes ______.
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
While isolating DNA from bacteria, which of the following enzymes is not required?
Would you choose an exonuclease while producing a recombinant DNA molecule?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
