Advertisements
Advertisements
Question
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Advertisements
Solution
Sticky ends are produced by restriction enzymes. These enzymes cut the strand of DNA a little away from the centre of the palindrome sites but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called 'sticky ends' on each strand.
These are called sticky ends because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase.
RELATED QUESTIONS
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
While isolating DNA from bacteria, which of the following enzymes is not required?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
How does one visualise DNA on an agarose gel?
Identify the activity of endonuclease and exonuclease in the given image.
How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?
Why is it mandatory to cut both the vector DNA and the foreign source DNA with the exact same restriction enzyme when constructing recombinant DNA?
According to the sieving effect of an agarose gel matrix, which DNA fragments move the farthest and fastest from the loading wells?
