Advertisements
Advertisements
Question
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Advertisements
Solution
Sticky ends are produced by restriction enzymes. These enzymes cut the strand of DNA a little away from the centre of the palindrome sites but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called 'sticky ends' on each strand.
These are called sticky ends because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase.
RELATED QUESTIONS
Explain with the help of a suitable example the naming of a restriction endonuclease.
Mention the difference in the mode of action of exonuclease and endonuclease.
Suggest a technique to a researcher who needs to separate fragments of DNA.
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
Molecular scissors, which cut DNA at specific site is ______.
'Restriction' in restriction enzyme refers to
Which of the following statements does not hold true for restriction enzyme?
Would you choose an exonuclease while producing a recombinant DNA molecule?
What is elution?
