Advertisements
Advertisements
Question
Give a reason why :
Single cloning site is preferred in a vector.
Advertisements
Solution
Cloning sites are short segments of DNA that contain sites for restriction enzymes. Single cloning site is preferred in a vector because multiple cloning sites would result in the generation of several fragments thus complicating the process.
APPEARS IN
RELATED QUESTIONS
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Explain briefly:
Restriction enzymes and DNA
Distinguish between exonuclease and endonuclease.
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
Would you choose an exonuclease while producing a recombinant DNA molecule?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel

How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?
In the standard nomenclature convention for restriction endonucleases, what do the second and third letters signify?
During agarose gel electrophoresis, why do DNA fragments migrate toward the anode (positive electrode)?
