Advertisements
Advertisements
Question
Give a reason why :
Single cloning site is preferred in a vector.
Advertisements
Solution
Cloning sites are short segments of DNA that contain sites for restriction enzymes. Single cloning site is preferred in a vector because multiple cloning sites would result in the generation of several fragments thus complicating the process.
APPEARS IN
RELATED QUESTIONS
Suggest a technique to a researcher who needs to separate fragments of DNA.
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Distinguish between exonuclease and endonuclease.
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
Restriction enzymes ______.
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
State the importance of elution in this process.
How are DNA fragments visualised once they are separated by gel electrophoresis?
