Question

In the restriction endonuclease EcoRI, what do the letter 'R' and the Roman numeral 'I' represent?

Options

• 'R' represents the strain RY13, and 'I' indicates that it was the first enzyme identified from this strain

• 'R' represents the genus \textit{Rhodococcus}, and 'I' denotes Type I restriction class

• 'R' represents the resistance plasmid, and 'I' indicates isolation from lane 1 of an electrophoresis gel

• 'R' represents recombinant origin, and 'I' denotes a single recognition site

Answer 1

In EcoRI, 'E' comes from the genus Escherichia, 'co' from the species coli, 'R' from the strain RY13, and 'I' denotes that it was the first enzyme identified from this specific strain.