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Question
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
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Solution
For genetic engineering processes, it is important that the isolated DNA is free of any other biomolecules and is in its purest form.
The DNA is intertwined around basic proteins called histones. During isolation of the DNA, it is treated with proteases (enzymes which degrade proteins) to remove any such proteins which may interfere with the genetic engineering processes.
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5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
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5’... ATC GTA AAG CTT CAT…3’
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