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प्रश्न
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
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उत्तर
For genetic engineering processes, it is important that the isolated DNA is free of any other biomolecules and is in its purest form.
The DNA is intertwined around basic proteins called histones. During isolation of the DNA, it is treated with proteases (enzymes which degrade proteins) to remove any such proteins which may interfere with the genetic engineering processes.
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संबंधित प्रश्न
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
What is PCR? Explain different steps involved in it.
From the following identify the methods of electrophoresis.
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
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DNA fingerprinting refers to ______.
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Write steps involved in gene amplification.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
