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प्रश्न
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
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उत्तर
For genetic engineering processes, it is important that the isolated DNA is free of any other biomolecules and is in its purest form.
The DNA is intertwined around basic proteins called histones. During isolation of the DNA, it is treated with proteases (enzymes which degrade proteins) to remove any such proteins which may interfere with the genetic engineering processes.
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संबंधित प्रश्न
Distinguish between plasmid DNA and chromosomal DNA.
Enlist and write in brief about the different biological tools required in rDNA technology.
Ti plasmid being used for introducing genes in plants obtained from ______.
What is PCR? Explain different steps involved in it.
Nucleic acid segment tagged with a radioactive molecule is called ______.
Name two restriction enzymes used in PCR.
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
What are the different types of electrophoresis?
Write a short note on vectors.
Which one of the following enzymes is used to join DNA fragments?
