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प्रश्न
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
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उत्तर
For genetic engineering processes, it is important that the isolated DNA is free of any other biomolecules and is in its purest form.
The DNA is intertwined around basic proteins called histones. During isolation of the DNA, it is treated with proteases (enzymes which degrade proteins) to remove any such proteins which may interfere with the genetic engineering processes.
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संबंधित प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
Expand the following abbreviation: BAC
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
______ is the soil bacterium that causes crown gall characterized by tumors in plants.
______ is a Type I restriction enzyme.
The gene transfer between unrelated plants is brought about by ____________.
Exonuclease performs the function of ____________.
EcoRl & Hind III are the examples of ______
Annealing step of PCR operates at ______ °C.
