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प्रश्न
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
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उत्तर
For genetic engineering processes, it is important that the isolated DNA is free of any other biomolecules and is in its purest form.
The DNA is intertwined around basic proteins called histones. During isolation of the DNA, it is treated with proteases (enzymes which degrade proteins) to remove any such proteins which may interfere with the genetic engineering processes.
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संबंधित प्रश्न
Give one significant difference between:
Electroporation and Gene Gun.
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
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Identify the characterization techniques that are based on molecular weights of compounds.
From the following identify the methods of electrophoresis.
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
From which of the following organisms the enzyme Taq polymerase used in PCR technique is obtained?
Which of the following viruses has the capacity to produce lysozymes?
Which of the following is not required in the preparation of a recombinant DNA molecule?
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
