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प्रश्न
Give a reason why :
Single cloning site is preferred in a vector.
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उत्तर
Cloning sites are short segments of DNA that contain sites for restriction enzymes. Single cloning site is preferred in a vector because multiple cloning sites would result in the generation of several fragments thus complicating the process.
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संबंधित प्रश्न
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
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Molecular scissors, which cut DNA at specific site is ______.
Would you choose an exonuclease while producing a recombinant DNA molecule?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
State the importance of elution in this process.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
Identify the activity of endonuclease and exonuclease in the given image.
