हिंदी

Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.

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प्रश्न

Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.

अति संक्षिप्त उत्तर
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उत्तर

Lysozyme is used for the isolation of DNA from bacterial cells, chitinase is used for the isolation of DNA from fungal cells and cellulase is used for the isolation of DNA from plant cells.

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अध्याय 11: Principles and Processes of Biotechnology - Test Your Progress [पृष्ठ ५३२]

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नूतन Biology [English] Class 12 ISC
अध्याय 11 Principles and Processes of Biotechnology
Test Your Progress | Q 8. | पृष्ठ ५३२

संबंधित प्रश्न

How are 'sticky ends' formed on a DNA strand? Why are they so called? 


Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?


Suggest a technique to a researcher who needs to separate fragments of DNA.


Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease


Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.


Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.


Give a reason why :
Single cloning site is preferred in a vector.


The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.

Applying Chargaff’s rule, what conclusion can be drawn?


A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.


Which of the following radioisotope is not suitable for DNA labeling based studies?


Molecular scissors, which cut DNA at specific site is ______.


'Restriction' in restriction enzyme refers to


The role of DNA ligase in the construction of a recombinant DNA molecule is ______.


Which of the following statements does not hold true for restriction enzyme?


Would you choose an exonuclease while producing a recombinant DNA molecule?


A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?


Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.

(a) What result will be obtained on staining with ethidium bromide? Explain with reason.

(b) The above setup was modified and a band with 250 base pairs was obtained at X.

What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?


What is elution?


Identify the activity of endonuclease and exonuclease in the given image.


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