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प्रश्न
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
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उत्तर
The palindromic sequences i.e. the sequence of base pairs read the same on both the DNA strands when the orientation of reading is kept the same, eg :
5' —— GAATTC —— 3'
3' —— CTTAAG —— 5'
- Every endonuclease inspects the entire DNA sequence for the palindromic recognition sequence.
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Explain briefly:
Restriction enzymes and DNA
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
Would you choose an exonuclease while producing a recombinant DNA molecule?
Identify the activity of endonuclease and exonuclease in the given image.
In the restriction endonuclease EcoRI, what do the letter 'R' and the Roman numeral 'I' represent?
Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?
