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प्रश्न
Which of the following statements does not hold true for restriction enzyme?
विकल्प
It recognises a palindromic nucleotide sequence
It is an endonuclease
It is isolated from viruses
It can produce the same kind of sticky ends in different DNA molecules
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उत्तर
It is isolated from viruses
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Suggest a technique to a researcher who needs to separate fragments of DNA.
Distinguish between exonuclease and endonuclease.
How does restriction endonuclease function?
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
DNA fragments separate according to size through?
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
'Restriction' in Restriction enzyme refers to ______.
Would you choose an exonuclease while producing a recombinant DNA molecule?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
Identify the activity of endonuclease and exonuclease in the given image.
Which of the following was the very first restriction endonuclease to be isolated and characterized?
How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?
In the standard nomenclature convention for restriction endonucleases, what do the second and third letters signify?
During agarose gel electrophoresis, why do DNA fragments migrate toward the anode (positive electrode)?
How are separated DNA bands visualized following agarose gel electrophoresis?
Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?
