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कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Which of the following statements does not hold true for restriction enzyme?

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प्रश्न

Which of the following statements does not hold true for restriction enzyme?

विकल्प

  • It recognises a palindromic nucleotide sequence

  • It is an endonuclease

  • It is isolated from viruses

  • It can produce the same kind of sticky ends in different DNA molecules

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उत्तर

It is isolated from viruses

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अध्याय 11: Biotechnology : Principles and Processes - MULTIPLE CHOICE QUESTIONS [पृष्ठ ७८]

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एनसीईआरटी एक्झांप्लर Biology Exemplar [English] Class 12
अध्याय 11 Biotechnology : Principles and Processes
MULTIPLE CHOICE QUESTIONS | Q 19. | पृष्ठ ७८

संबंधित प्रश्न

Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?


How does a restriction nuclease function? Explain


Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.


Which of the following radioisotope is not suitable for DNA labeling based studies?


A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______


In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.


The role of DNA ligase in the construction of a recombinant DNA molecule is ______.


What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?


Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?


A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?


What is elution?


Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.

\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]

\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]

Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.


Identify the activity of endonuclease and exonuclease in the given image.


What is the precise definition of a palindromic sequence in DNA?


In the restriction endonuclease EcoRI, what do the letter 'R' and the Roman numeral 'I' represent?


Why is it mandatory to cut both the vector DNA and the foreign source DNA with the exact same restriction enzyme when constructing recombinant DNA?


During agarose gel electrophoresis, why do DNA fragments migrate toward the anode (positive electrode)?


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