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प्रश्न
How does one visualise DNA on an agarose gel?
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उत्तर
A compound called Ethidium Bromide stains DNA, which on irradiating with Ultraviolet, fluoresce gives orange light. Hence, DNA fragments appear as orange band in the presence of Ethidium Bromide and UV.
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संबंधित प्रश्न
Do eukaryotic cells have restriction endonucleases? Justify your answer.
How does restriction endonuclease function?
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
Molecular scissors, which cut DNA at specific site is ______.
'Restriction' in restriction enzyme refers to
DNA fragments separate according to size through?
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
While isolating DNA from bacteria, which of the following enzymes is not required?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
How are DNA fragments visualised once they are separated by gel electrophoresis?
