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प्रश्न
How does one visualise DNA on an agarose gel?
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उत्तर
A compound called Ethidium Bromide stains DNA, which on irradiating with Ultraviolet, fluoresce gives orange light. Hence, DNA fragments appear as orange band in the presence of Ethidium Bromide and UV.
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संबंधित प्रश्न
Mention the difference in the mode of action of exonuclease and endonuclease.
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
How does restriction endonuclease function?
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
State the importance of elution in this process.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
State the principle involved in separation of DNA fragments using gel electrophoresis.
Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of ______.
What is the precise definition of a palindromic sequence in DNA?
In the standard nomenclature convention for restriction endonucleases, what do the second and third letters signify?
Why is it mandatory to cut both the vector DNA and the foreign source DNA with the exact same restriction enzyme when constructing recombinant DNA?
What is the origin of agarose, the matrix most commonly used in gel electrophoresis?
