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प्रश्न
Would you choose an exonuclease while producing a recombinant DNA molecule?
Would you like to choose an exonuclease enzyme while producing a recombinant DNA molecule?
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उत्तर
No, as exonuclease acts on the free ends of linear DNA molecule. Therefore, instead of producing DNA fragments with sticky ends, it will shorten or completely degrade the DNA fragment containing the gene of interest, and the circular plasmid (vector) will not get cut as it lacks free ends.
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संबंधित प्रश्न
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Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
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'Restriction' in restriction enzyme refers to
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- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
Identify the activity of endonuclease and exonuclease in the given image.
Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of ______.
Which of the following was the very first restriction endonuclease to be isolated and characterized?
In the standard nomenclature convention for restriction endonucleases, what do the second and third letters signify?
In the restriction endonuclease EcoRI, what do the letter 'R' and the Roman numeral 'I' represent?
Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?
During agarose gel electrophoresis, why do DNA fragments migrate toward the anode (positive electrode)?
What is the process called when desired, separated DNA bands are physically cut out from the agarose gel and extracted from the gel piece?
