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प्रश्न
Would you choose an exonuclease while producing a recombinant DNA molecule?
Would you like to choose an exonuclease enzyme while producing a recombinant DNA molecule?
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उत्तर
No, as exonuclease acts on the free ends of linear DNA molecule. Therefore, instead of producing DNA fragments with sticky ends, it will shorten or completely degrade the DNA fragment containing the gene of interest, and the circular plasmid (vector) will not get cut as it lacks free ends.
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Do eukaryotic cells have restriction endonucleases? Justify your answer.
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Give a reason why :
Single cloning site is preferred in a vector.
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
Molecular scissors, which cut DNA at specific site is ______.
DNA fragments separate according to size through?
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
'Restriction' in Restriction enzyme refers to ______.
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
State the principle involved in separation of DNA fragments using gel electrophoresis.
Identify the activity of endonuclease and exonuclease in the given image.
