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प्रश्न
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
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उत्तर
The DNA strand is cut by restriction enzymes between the similar two bases on the opposing strands, but just outside of the palindromic sites: The enzyme cuts both the foreign DNA and the vector DNA similarly. Only when the DNA sequence GAATTC is present does EcoRI cut the DNA between the nucleotides G and A. As a result, there are single-stranded, overhanging lengths at the end that are known to as sticky ends. Hydrogen bonds are formed between sticky ends and their complementary sliced opposites. The DNA ligase enzyme's function is made easier by the end's stickiness. Recombinant DNA was created as a result.
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संबंधित प्रश्न
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Restriction enzymes ______.
DNA fragments separate according to size through?
Which of the following bacteria is not a source of restriction endonuclease?
Would you choose an exonuclease while producing a recombinant DNA molecule?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
What is elution?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
State the principle involved in separation of DNA fragments using gel electrophoresis.
