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प्रश्न
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
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उत्तर
The DNA strand is cut by restriction enzymes between the similar two bases on the opposing strands, but just outside of the palindromic sites: The enzyme cuts both the foreign DNA and the vector DNA similarly. Only when the DNA sequence GAATTC is present does EcoRI cut the DNA between the nucleotides G and A. As a result, there are single-stranded, overhanging lengths at the end that are known to as sticky ends. Hydrogen bonds are formed between sticky ends and their complementary sliced opposites. The DNA ligase enzyme's function is made easier by the end's stickiness. Recombinant DNA was created as a result.
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संबंधित प्रश्न
Suggest a technique to a researcher who needs to separate fragments of DNA.
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
Which of the following bacteria is not a source of restriction endonuclease?
Would you choose an exonuclease while producing a recombinant DNA molecule?
What is elution?
State the importance of elution in this process.
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of ______.
