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Question
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
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Solution
The DNA strand is cut by restriction enzymes between the similar two bases on the opposing strands, but just outside of the palindromic sites: The enzyme cuts both the foreign DNA and the vector DNA similarly. Only when the DNA sequence GAATTC is present does EcoRI cut the DNA between the nucleotides G and A. As a result, there are single-stranded, overhanging lengths at the end that are known to as sticky ends. Hydrogen bonds are formed between sticky ends and their complementary sliced opposites. The DNA ligase enzyme's function is made easier by the end's stickiness. Recombinant DNA was created as a result.
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RELATED QUESTIONS
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
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(b) The above setup was modified and a band with 250 base pairs was obtained at X.
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'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
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How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?
Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?
