Advertisements
Advertisements
प्रश्न
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
Advertisements
उत्तर
The DNA strand is cut by restriction enzymes between the similar two bases on the opposing strands, but just outside of the palindromic sites: The enzyme cuts both the foreign DNA and the vector DNA similarly. Only when the DNA sequence GAATTC is present does EcoRI cut the DNA between the nucleotides G and A. As a result, there are single-stranded, overhanging lengths at the end that are known to as sticky ends. Hydrogen bonds are formed between sticky ends and their complementary sliced opposites. The DNA ligase enzyme's function is made easier by the end's stickiness. Recombinant DNA was created as a result.
APPEARS IN
संबंधित प्रश्न
How does restriction endonuclease function?
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
'Restriction' in Restriction enzyme refers to ______.
Which of the following statements does not hold true for restriction enzyme?
Would you choose an exonuclease while producing a recombinant DNA molecule?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
