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प्रश्न
'Restriction' in Restriction enzyme refers to ______.
पर्याय
Cleaving of phosphodiester bond in DNA by the enzyme
Cutting of DNA at specific position only
Prevention of the multiplication of bacteriophage by the host bacteria
All of the above
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उत्तर
'Restriction' in Restriction enzyme refers to prevention of the multiplication of bacteriophage by the host bacteria.
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संबंधित प्रश्न
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Explain briefly:
Restriction enzymes and DNA
How does restriction endonuclease function?
Give a reason why :
Single cloning site is preferred in a vector.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
Which of the following radioisotope is not suitable for DNA labeling based studies?
Restriction enzymes ______.
'Restriction' in restriction enzyme refers to
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
While isolating DNA from bacteria, which of the following enzymes is not required?
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
How are DNA fragments visualised once they are separated by gel electrophoresis?
Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of ______.
How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?
Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?
How are separated DNA bands visualized following agarose gel electrophoresis?
Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?
