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कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

'Restriction' in Restriction enzyme refers to ______.

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प्रश्न

'Restriction' in Restriction enzyme refers to ______.

पर्याय

  • Cleaving of phosphodiester bond in DNA by the enzyme

  • Cutting of DNA at specific position only

  • Prevention of the multiplication of bacteriophage by the host bacteria

  • All of the above

MCQ
रिकाम्या जागा भरा
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उत्तर

'Restriction' in Restriction enzyme refers to prevention of the multiplication of bacteriophage by the host bacteria.

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पाठ 11: Biotechnology : Principles and Processes - MULTIPLE CHOICE QUESTIONS [पृष्ठ ७६]

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एनसीईआरटी एक्झांप्लर Biology Exemplar [English] Class 12
पाठ 11 Biotechnology : Principles and Processes
MULTIPLE CHOICE QUESTIONS | Q 5. | पृष्ठ ७६

संबंधित प्रश्‍न

How are 'sticky ends' formed on a DNA strand? Why are they so called? 


Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?


Suggest a technique to a researcher who needs to separate fragments of DNA.


Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.


Distinguish between exonuclease and endonuclease.


Explain the roles of the following with the help of an example each in recombinant DNA technology :

Restriction Enzymes


The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.

Applying Chargaff’s rule, what conclusion can be drawn?


There is a restriction endonudease called as EcoRI. What does co part in it stands for?


Molecular scissors, which cut DNA at specific site is ______.


DNA fragments separate according to size through?


Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?


While isolating DNA from bacteria, which of the following enzymes is not required?


Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.


A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?


CTTAAG
GAATTC

  1. What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
  2. What is their significance in biotechnology?

What is elution?


'EcoRI' has played a very significant role in rDNA technology.

  1. Explain the convention for naming EcoRI.
  2. Write the recognition site and the cleavage sites of this restriction endonuclease.

How are DNA fragments visualised once they are separated by gel electrophoresis?


Identify the activity of endonuclease and exonuclease in the given image.


Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of ______.


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