Advertisements
Advertisements
प्रश्न
'Restriction' in Restriction enzyme refers to ______.
पर्याय
Cleaving of phosphodiester bond in DNA by the enzyme
Cutting of DNA at specific position only
Prevention of the multiplication of bacteriophage by the host bacteria
All of the above
Advertisements
उत्तर
'Restriction' in Restriction enzyme refers to prevention of the multiplication of bacteriophage by the host bacteria.
APPEARS IN
संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Mention the difference in the mode of action of exonuclease and endonuclease.
Suggest a technique to a researcher who needs to separate fragments of DNA.
How does restriction endonuclease function?
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
Molecular scissors, which cut DNA at specific site is ______.
'Restriction' in restriction enzyme refers to
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
Identify the activity of endonuclease and exonuclease in the given image.
In the standard nomenclature convention for restriction endonucleases, what do the second and third letters signify?
Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?
During agarose gel electrophoresis, why do DNA fragments migrate toward the anode (positive electrode)?
