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प्रश्न
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
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उत्तर
The reasons are as follows:
- DNA sample that was loaded on the gel may have got contaminated with nuclease (exo-or endo-or both) and completely degraded.
- Electrodes were put in opposite orientation in the gel assembly that is anode towards the wells (where DNA sample is loaded). Since DNA molecules are negatively charged, they move towards anode and hence move out of the gel instead of moving into the matrix of gel.
- Ethidium bromide was not added at all or was not added in sufficient concentration and DNA was not visible.
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संबंधित प्रश्न
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Mention the difference in the mode of action of exonuclease and endonuclease.
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
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Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
Which of the following radioisotope is not suitable for DNA labeling based studies?
Restriction enzymes ______.
DNA fragments separate according to size through?
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Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
'Restriction' in Restriction enzyme refers to ______.
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
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State the importance of elution in this process.
