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प्रश्न
Describe the role of CaCl2 in the preparation of competent cells?
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उत्तर
CaCl2 is known to increase the efficiency of DNA uptake to produce transformed bactrial cells. The divalent Ca+2 ions supposedly create transient pores on the bactrial cell wall by which the entry of foreign DNA is facilitated into the bactrial cells.
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
Why is the coding sequence of an enzyme β-galactosidase a preferred selectable marker in comparison to the ones named above?
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of ______.
Which of the following is correctly matched?
Which of the following is not a genetic vector?
What does ‘competent’ refer to in competent cells used in transformation experiments?
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
The following diagram showing restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’ genes:
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
Which of the following is not a cloning vector?
What is the primary function of a vector in recombinant DNA technology?
What is the basic biological nature of plasmids when used as cloning vectors?
Which of the following serves as an example of selectable markers commonly found in plasmid vectors like pBR322?
What is the primary role of a cloning site in a vector?
In blue–white screening, what do the white colonies indicate?
Which vector derived from \(\textit{Agrobacterium tumefaciens}\) is utilized for delivering foreign genes into plants?
