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प्रश्न
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
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उत्तर १
In the cloning vector pBR322, ampicillin and tetracycline resistance genes serve as selectable markers. They help in the selection of transformed cells from nontransformed cells. They also help distinguish recombinant cells from nonrecombinant cells.
उत्तर २
The selectable markers in the cloning vector pBR322 are the genes encoding resistance to antibiotics, specifically ampicillin and tetracycline. These markers play a crucial role in identifying and selecting transformants. When a foreign DNA is inserted into the vector, it disrupts one of the antibiotic resistance genes, often the tetracycline resistance gene. This results in transformants that are resistant to ampicillin but sensitive to tetracycline, allowing selection of recombinant cells. The markers enable growth of only those bacteria that have taken up the plasmid and help distinguish recombinants from non-recombinants through antibiotic sensitivity patterns.
संबंधित प्रश्न
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
Describe briefly the following:
Origin of replication
Write the role of ‘restriction sites’ in the cloning vector pBR322.
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
A clone is a ______.
Which of the following is correctly matched?
Which of the following statement is not true for a clone?
Plasmids are suitable vectors for gene cloning because these ______.
Describe the role of CaCl2 in the preparation of competent cells?
Name the regions marked A, B and C.
