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प्रश्न
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
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उत्तर
Agrobacterium tumefaciens harbours a megaplasmid called Ti-plasmid. This has a T-DNA region flanked by the left border and right border sequence. The T-DNA gets transferred and integrates with the host plant DNA. This property of Ti-plasmid has been exploited for cloning gene of interest and stably integrating them into the plant genese. Therefore, by using Ti-plasmid or its derivatives, recombinant plant cells with desired genes of interest stably integrated in the plant genome have been successfully produced.
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संबंधित प्रश्न
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?
Describe briefly the following:
Origin of replication
Why is the coding sequence of an enzyme β-galactosidase a preferred selectable marker in comparison to the ones named above?
Write the role of ‘restriction sites’ in the cloning vector pBR322.
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
Which of the following is correctly matched?
Which of the following statement is not true for a clone?
A suitable vector for gene cloning in higher organism is ______.
Plasmids are suitable vectors for gene cloning because these ______.
Which of the following is not a genetic vector?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
Which of the following is not a cloning vector?
What is the primary function of a vector in recombinant DNA technology?
Which of the following serves as an example of selectable markers commonly found in plasmid vectors like pBR322?
What is the primary role of a cloning site in a vector?
Which of the following is a group of vectors used for gene transfer into animals?
