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Question
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
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Solution
Agrobacterium tumefaciens harbours a megaplasmid called Ti-plasmid. This has a T-DNA region flanked by the left border and right border sequence. The T-DNA gets transferred and integrates with the host plant DNA. This property of Ti-plasmid has been exploited for cloning gene of interest and stably integrating them into the plant genese. Therefore, by using Ti-plasmid or its derivatives, recombinant plant cells with desired genes of interest stably integrated in the plant genome have been successfully produced.
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RELATED QUESTIONS
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
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Choose the correct answer from the options given below:
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
What is the primary role of a cloning site in a vector?
In blue–white screening, what do the white colonies indicate?
Which vector derived from \(\textit{Agrobacterium tumefaciens}\) is utilized for delivering foreign genes into plants?
