Advertisements
Advertisements
Question
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Advertisements
Solution
Agrobacterium is a bacterium that infects plant tissues by transferring its plasmid T-DNA to the plant genome. This property of Agrobacterium is exploited in the biotechnology industry.
The desired gene that has to be to transferred to a particular plant is inserted in a plasmid T-DNA of Agrobacterium. Then, this engineered Agrobacterium is allowed to infect that particular plant, so that it can transfer the desired gene to the plant genome through its T-DNA. In this way, the desired changes are made in the plant.
APPEARS IN
RELATED QUESTIONS
Describe briefly the following:
Origin of replication
Write the role of ‘restriction sites’ in the cloning vector pBR322.
What is ‘Ori’ ? State its importance during cloning of a vector.
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of ______.
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
Describe the role of CaCl2 in the preparation of competent cells?
Name the regions marked A, B and C.

For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
The figure below shows the structure of a plasmid.

A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
