Advertisements
Advertisements
Question
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Advertisements
Solution
Agrobacterium is a bacterium that infects plant tissues by transferring its plasmid T-DNA to the plant genome. This property of Agrobacterium is exploited in the biotechnology industry.
The desired gene that has to be to transferred to a particular plant is inserted in a plasmid T-DNA of Agrobacterium. Then, this engineered Agrobacterium is allowed to infect that particular plant, so that it can transfer the desired gene to the plant genome through its T-DNA. In this way, the desired changes are made in the plant.
APPEARS IN
RELATED QUESTIONS
Name the selectable markers in the cloning vector pBR322. Mention the role they play.
What is ‘Ori’ ? State its importance during cloning of a vector.
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain.
Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
The “Ti plasmid” of Agrobacterium tumefaciens stands for ______.
Which of the following serves as an example of selectable markers commonly found in plasmid vectors like pBR322?
What is the primary role of a cloning site in a vector?
In blue–white screening, what do the white colonies indicate?
