Advertisements
Advertisements
Question
Explain with the help of a suitable example the naming of a restriction endonuclease.
Advertisements
Solution
The naming of a restriction endonuclease is done in the following way:
-
Ist letter represents the genus of the organism from which the enzyme is derived.
-
IInd and IIIrd letters represent the species of the organism.
-
IVth letter represents the name of the strain.
-
Roman number represents order of isolation.
For example, In EcoRI − Derived from E.coli, strain R.
It is the Ist to be discovered.
RELATED QUESTIONS
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
Restriction enzymes ______.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
While isolating DNA from bacteria, which of the following enzymes is not required?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
State the importance of elution in this process.
How are DNA fragments visualised once they are separated by gel electrophoresis?
In the standard nomenclature convention for restriction endonucleases, what do the second and third letters signify?
In the restriction endonuclease EcoRI, what do the letter 'R' and the Roman numeral 'I' represent?
