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Question
Explain with the help of a suitable example the naming of a restriction endonuclease.
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Solution
The naming of a restriction endonuclease is done in the following way:
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Ist letter represents the genus of the organism from which the enzyme is derived.
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IInd and IIIrd letters represent the species of the organism.
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IVth letter represents the name of the strain.
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Roman number represents order of isolation.
For example, In EcoRI − Derived from E.coli, strain R.
It is the Ist to be discovered.
RELATED QUESTIONS
How does a restriction nuclease function? Explain
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
Molecular scissors, which cut DNA at specific site is ______.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
Which of the following statements does not hold true for restriction enzyme?
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
During agarose gel electrophoresis, why do DNA fragments migrate toward the anode (positive electrode)?
Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?
