Advertisements
Advertisements
Question
Explain with the help of a suitable example the naming of a restriction endonuclease.
Advertisements
Solution
The naming of a restriction endonuclease is done in the following way:
-
Ist letter represents the genus of the organism from which the enzyme is derived.
-
IInd and IIIrd letters represent the species of the organism.
-
IVth letter represents the name of the strain.
-
Roman number represents order of isolation.
For example, In EcoRI − Derived from E.coli, strain R.
It is the Ist to be discovered.
RELATED QUESTIONS
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
How does restriction endonuclease function?
Give a reason why :
Single cloning site is preferred in a vector.
Which of the following radioisotope is not suitable for DNA labeling based studies?
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
Which of the following statements does not hold true for restriction enzyme?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
Identify the activity of endonuclease and exonuclease in the given image.
