Advertisements
Advertisements
Question
State the importance of elution in this process.
Advertisements
Solution
The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with cloning vectors.
APPEARS IN
RELATED QUESTIONS
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Distinguish between exonuclease and endonuclease.
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
DNA fragments separate according to size through?
'Restriction' in Restriction enzyme refers to ______.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
Which of the following statements does not hold true for restriction enzyme?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.

(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.

What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
