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प्रश्न
State the importance of elution in this process.
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उत्तर
The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with cloning vectors.
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
How does a restriction nuclease function? Explain
Distinguish between exonuclease and endonuclease.
Which of the following radioisotope is not suitable for DNA labeling based studies?
DNA fragments separate according to size through?
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
