Advertisements
Advertisements
प्रश्न
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
Advertisements
उत्तर
H’ from name of genus Haemophilus, ‘d’ from name of strain Rd. TIT indicates the order in which the enzyme was isolated from the Rd strain of bacteria.
APPEARS IN
संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Mention the difference in the mode of action of exonuclease and endonuclease.
Suggest a technique to a researcher who needs to separate fragments of DNA.
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Distinguish between exonuclease and endonuclease.
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Which of the following radioisotope is not suitable for DNA labeling based studies?
'Restriction' in restriction enzyme refers to
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
State the importance of elution in this process.
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
State the principle involved in separation of DNA fragments using gel electrophoresis.
Identify the activity of endonuclease and exonuclease in the given image.
