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प्रश्न
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
Restriction enzymes present in the cloning site of a vector should not have more than one recognition site. Comment.
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उत्तर
If the restriction enzymes have more than one recognition site in a vector then the vector itself will get fragmented on treatment with the restriction enzyme.
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संबंधित प्रश्न
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Mention the difference in the mode of action of exonuclease and endonuclease.
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Do eukaryotic cells have restriction endonucleases? Justify your answer.
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Distinguish between exonuclease and endonuclease.
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
Restriction enzymes ______.
Molecular scissors, which cut DNA at specific site is ______.
DNA fragments separate according to size through?
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
While isolating DNA from bacteria, which of the following enzymes is not required?
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
Which of the following statements does not hold true for restriction enzyme?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
How does one visualise DNA on an agarose gel?
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
How are DNA fragments visualised once they are separated by gel electrophoresis?
Identify the activity of endonuclease and exonuclease in the given image.
