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प्रश्न
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
Restriction enzymes present in the cloning site of a vector should not have more than one recognition site. Comment.
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उत्तर
If the restriction enzymes have more than one recognition site in a vector then the vector itself will get fragmented on treatment with the restriction enzyme.
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\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
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