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प्रश्न
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
Restriction enzymes present in the cloning site of a vector should not have more than one recognition site. Comment.
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उत्तर
If the restriction enzymes have more than one recognition site in a vector then the vector itself will get fragmented on treatment with the restriction enzyme.
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संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Mention the difference in the mode of action of exonuclease and endonuclease.
Suggest a technique to a researcher who needs to separate fragments of DNA.
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Distinguish between exonuclease and endonuclease.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Give a reason why :
Single cloning site is preferred in a vector.
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
Which of the following radioisotope is not suitable for DNA labeling based studies?
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
Molecular scissors, which cut DNA at specific site is ______.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
'Restriction' in Restriction enzyme refers to ______.
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
How does one visualise DNA on an agarose gel?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?
What is the origin of agarose, the matrix most commonly used in gel electrophoresis?
Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?
