Advertisements
Advertisements
प्रश्न
Identify the activity of endonuclease and exonuclease in the given image.
पर्याय
Endonuclease Exonuclease 
Endonuclease Exonuclease 
Endonuclease Exonuclease 
Endonuclease Exonuclease 
Advertisements
उत्तर
| Endonuclease | Exonuclease |
|
|
Explanation:
Endonucleases are a type of restriction enzyme that cuts the DNA at a specified location. It is widely used to clip the sequence in order to obtain DNA fragments with sticky ends. Sticky ends are single-stranded DNA segments that can establish hydrogen bonds with adjacent cut DNA segments. The enzyme ligase later joins these ends. Exonuclease is a restriction enzyme that removes nucleotides from the 5' and 3' ends of DNA strands.
APPEARS IN
संबंधित प्रश्न
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Distinguish between exonuclease and endonuclease.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
What is elution?
Which of the following was the very first restriction endonuclease to be isolated and characterized?
In the restriction endonuclease EcoRI, what do the letter 'R' and the Roman numeral 'I' represent?
During agarose gel electrophoresis, why do DNA fragments migrate toward the anode (positive electrode)?
Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?
