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प्रश्न
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
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उत्तर
Gel electrophoresis is a technique of separating DNA fragments formed by the action of restriction endonucleases.
The fragments of DNA are placed in a typical agarose gel under an electric field. The DNA fragments move towards the anode as these fragments are negatively charged molecules. The DNA fragments separate according to their size through the sieving effect provided by the agarose gel. The smaller the fragment size, the farther it moves. The separated DNA fragments are stained with ethidium bromide followed by exposure to ultraviolet radiation. The DNA fragments are seen as orangecoloured and are cut out from the agarose gel and extracted from the gel piece. This step is called elution.
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संबंधित प्रश्न
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Give a reason why :
Single cloning site is preferred in a vector.
Which of the following radioisotope is not suitable for DNA labeling based studies?
While isolating DNA from bacteria, which of the following enzymes is not required?
Which of the following bacteria is not a source of restriction endonuclease?
Which of the following statements does not hold true for restriction enzyme?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
State the importance of elution in this process.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
State the principle involved in separation of DNA fragments using gel electrophoresis.
