Advertisements
Advertisements
प्रश्न
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Why is the enzyme cellulase used for isolating genetic material from plant cells but not for animal cells?
Advertisements
उत्तर
Plant cells have a cell wall made up of cellulose, whereasanimal cells lack cell wall and do not contain cellulose. Therefore, the enzyme cellulase is needed for isolating genetic material from plant cells only.
संबंधित प्रश्न
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
Restriction enzymes ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
State the principle involved in separation of DNA fragments using gel electrophoresis.
How are DNA fragments visualised once they are separated by gel electrophoresis?
Which of the following was the very first restriction endonuclease to be isolated and characterized?
In the standard nomenclature convention for restriction endonucleases, what do the second and third letters signify?
During agarose gel electrophoresis, why do DNA fragments migrate toward the anode (positive electrode)?
According to the sieving effect of an agarose gel matrix, which DNA fragments move the farthest and fastest from the loading wells?
Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?
