Advertisements
Advertisements
Questions
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Why is the enzyme cellulase used for isolating genetic material from plant cells but not for animal cells?
Advertisements
Solution
Plant cells have a cell wall made up of cellulose, whereasanimal cells lack cell wall and do not contain cellulose. Therefore, the enzyme cellulase is needed for isolating genetic material from plant cells only.
RELATED QUESTIONS
Mention the difference in the mode of action of exonuclease and endonuclease.
Suggest a technique to a researcher who needs to separate fragments of DNA.
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Distinguish between exonuclease and endonuclease.
How does restriction endonuclease function?
Give a reason why :
Single cloning site is preferred in a vector.
Which of the following radioisotope is not suitable for DNA labeling based studies?
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
Molecular scissors, which cut DNA at specific site is ______.
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
'Restriction' in Restriction enzyme refers to ______.
Would you choose an exonuclease while producing a recombinant DNA molecule?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel

What is elution?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
State the principle involved in separation of DNA fragments using gel electrophoresis.
Identify the activity of endonuclease and exonuclease in the given image.
